Mice have been the workhorse of a vast majority of discoveries related to drugs, therapies and treatments for a wide variety of diseases. Our understanding of human disease mechanisms is already pushing the limits of what the traditional lab mouse can provide. It is clear that in order to model human disease, we need to study it in a human system.
By carefully replacing and engrafting human cells into highly immunodeficient mice, we have successfully produced mice with completely functional human immune and liver systems.
These humanized mice unlock the possibility for studying many diseases that cannot be modelled in mouse systems such as Human immunodeficient virus infections, hepatitis infections as well as auto-immune disorders.
Humanized Mice
Humanized Mice Models
Human Immune System Mice (HiMice)
Our immune system humanized mice provide a fully functional human immune system within a murine host, offering unparalleled opportunities to study complex human immunology. These models are essential for evaluating the next generation of immunotherapies, vaccines, and treatments for autoimmune and infectious diseases.
Background: NOD-SCID-IL2rg -/-
Humanization method : CD34+ hematopoietic stem cells (HSCs)
Human Immune System:
hCD45 cells: 20-60% (blood); >80% (spleen)
hCD45 Absolute cell count: >300 cell/µL
T-cells: 20-60%
B-cells: 20-60%
Myeloid Cells: 0-5%
NK Cells: 0-5%
Applications: Immunotherapy, Immunotoxicity, Cytokine release syndrome, Autoimmune Disease, Oncology, Infectious diseases, Inflammation.
These models feature chimeric livers repopulated with functional human hepatocytes, creating a unique in vivo environment for studying liver-specific pathogens, drug-induced liver injury (DILI), and metabolic pathways.
Background: Fah –/– NOD-SCID-IL2rg -/-
Humanization method : Human Primary Hepatocytes
Human Immune System: N.A.
Liver: > 70% Human
GvHD: No
Applications: Hepatitis, Liver Cirrhosis, Non-alcoholic fatty liver disease (NAFLD), Non-alcoholic steatohepatitis (NASH), human-specific pharmacokinetics, toxicology and infectious diseases.
Human Liver Mice (HepMice)
PBMC mice are constructed from the engraftment of adult blood mononuclear cells, as opposed to hematopoietic cells. This model provides an accelerated immune testing platform since the immune system is fully developed after injection of PBMCs.
Background: NOD-SCID-IL2rg -/-
Humanization method : Peripheral Blood Mononuclear Cells (PBMCs)
Human Immune System:
hCD45 cells: 20-80% (blood)
hCD45 Absolute cell count: >100 cell/µL
T-cells: 80-100%
B-cells: 0-10%
Myeloid Cells: 0-5%
NK Cells: 0-5%
Applications: GvHD, Immuno-oncology, Immunology, Infectious diseases.