Customized Research
Core Facilities & Services
Cytokine quantification in peripheral blood and tissues (e.g. multiplex-bead assay)
Ex vivo immune cell culture assays (e.g. T cell activation assay, M1/M2 macrophage cytokine release assay)
ELISPOT (e.g. human and mouse IFNγ)
Gene expression (RT-PCR)
CORE SERVICE
Flow Cytometry
General introduction: Immune monitoring by flow cytometry is a fast and highly informative way of understanding the immune response to various applications such as drugs intervention in disease models or characterization of tumor infiltrating leukocytes in tumor microenvironment. We have optimized protocols for tissue processing to isolate immune cells and the capacity to customize flow cytometry panels to suit your needs.
Example of T cells analysis in spleen of HiMicE:
Table: List of common human and mouse immune cell markers tested previously
| Cell Types | Common human and mouse markers tested previously |
|---|---|
| T Lymphocytes and NK | CD3, CD4, CD8, CD25, CD127, CD45RA, CCR7, FoxP3, CD56, CD62L, CD44 |
| B Lymphocytes | CD19, CD20, IgM, IgD, IgG, CD38, CD10, CD24, CD138, CD27 |
| Myeloid cells | CD14, CD16, CD33, CD123, CD1c, CD11b, CD206, CD11c, Siglec-F, CD68, Ly6G, Ly6C |
| Activation & Exhaustion markers | HLADR, CD86, CD80, CD69, PD-1, CTLA-4 |
| Colon Length Reduction | Reduced in DSS treated mice which is proportional to % DSS administered. |
| Loss of Colon Integrity | Loss of colon integrity observed in DSS treated mice, more severe in 3% DSS compared to 1.5% DSS. Similar observations in DSS treated M-NSG indicating infiltration of mouse immune cells. |
| Colon Wall Thickening | Less obvious in DSS treated mice |
| Cell Infiltration | Increased cell infiltration in inter-spatial space of villi and colon. |
CORE SERVICE
Histopathology Analysis
Our histopathology capabilities include:
Tissue processing for paraffin and cryopreservation
Embedding and sectioning
Hematoxylin and eosin stain
Immunohistochemical stains
Immunofluorescence stains
Digitalization and image quantification
Example of IHC analysis on tumor from HiMice:
Example of H&E, Fast-Green Sirius Red and Oil-Red stain on liver from high-fat diet fed HiMice:
CORE SERVICE
In Vitro Assay
Our capabilities include:
Cytokine quantification in peripheral blood and tissues (e.g. multiplex-bead assay)
Ex vivo immune cell culture assays (e.g. T cell activation assay, M1/M2 macrophage cytokine release assay)
Elispot (e.g. human and mouse IFNγ)
Gene expression (RT-PCR)
Example of human IFN-γ ELISPOT assay on splenocytes from humanized mice
Example of cytokine profiling of in vitro polarized human macrophages by multiplex cytokine assay
Given the complexity of human disease, in-depth knowledge of each model is key to success in pre-clinical studies. Invivocue offers tailor-made study designs to suit your research needs.